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Oxidative and nitrosative stress in acute ischaemic stroke. |
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Oxidative and nitrosative stress in acute ischaemic stroke.
Ann Clin Biochem. 2007 Jan;44(Pt 1):43-7
Senes M, Kazan N, Coskun O, Zengi O, Inan L, Yucel D.
Department of Clinical Biochemistry, Ankara Education and Research Hospital, Cebeci, Ankara 06340, Turkey.
BACKGROUND:
Increased nitric oxide (NO) production may result in further brain
damage via nitric oxide synthase uncoupling in patients with acute
ischaemic stroke by increasing free radical formation and oxidative
stress. In this connection, we measured nitrite and nitrate (NO
metabolites), ischaemia-modified albumin (IMA) and thiobarbituric
acid-reactive substances (TBARS) in patients with ischaemic stroke.
METHODS: We studied 41 patients with ischaemic stroke (22 men and 19
women, aged 65+/-13 years) and 37 age- and sex-matched controls (22 men
and 15 women, aged 65+/-8 years). Blood samples were drawn within the
first 24 h from the onset of symptoms in the patient group. Fasting
morning samples were used in the control group. Concentrations of
nitrite and nitrate were determined by Griess reagent; concentrations
of IMA were determined by the albumin cobalt-binding test; and
concentrations of TBARS were determined colorimetrically by
thiobarbituric acid. RESULTS: Nitrate, IMA and TBARS concentrations
were significantly increased compared with controls (P<0.005,
P<0.001, and P=0.01, respectively). CONCLUSIONS: Patients with acute
ischaemic stroke exhibit abnormalities in a range of markers of
increased nitrosative and oxidative stress. These abnormalities may
contribute to greater brain damage in patients with acute ischaemic
stress.
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